Aplikasi Mikrobia Simbiotik Dalam Meningkatkan Keberhasilan Aklimatisasi Planlet Anggrek
Aklimatisasi, Bakteri Simbiotik, Dendrobium, PGPR, Planlet
Abstrak
Aklimatisasi merupakan tahap kritis dalam perbanyakan anggrek hasil kultur jaringan karena planlet harus beradaptasi dari kondisi in vitro ke lingkungan ex vitro. Pemanfaatan bakteri simbiotik berpotensi meningkatkan keberhasilan aklimatisasi melalui peningkatan penyerapan hara dan toleransi terhadap cekaman lingkungan. Penelitian ini bertujuan menganalisis pengaruh aplikasi bakteri simbiotik terhadap keberhasilan aklimatisasi planlet anggrek. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan konsentrasi bakteri simbiotik, yaitu tanpa bakteri (A0), 2% (A1), 4% (A2), 6% (A3), dan 8% (A4), masing-masing diulang sebanyak 10 kali. Parameter yang diamati meliputi tingkat kelangsungan hidup, tinggi planlet, panjang akar, dan kandungan klorofil total. Hasil penelitian menunjukkan bahwa aplikasi bakteri simbiotik berpengaruh nyata terhadap seluruh parameter pengamatan. Perlakuan A3 menghasilkan pertumbuhan dan tingkat kelangsungan hidup tertinggi, yaitu mencapai 90%.
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Referensi
Alpandari, H., Krestiani, V., Krestiani, V., Sari, S. I., & Sari, S. I. (2024). Respon Pertumbuhan Dan Hasil Tanaman Okra Hijau (Abelmoschus esculentus L.) Terhadap Konsentrasi Dan Frekuensi Plant Growth Promoting Rhizobacteria (PGPR). Muria Jurnal Agroteknologi (MJ-Agroteknologi), 3(1), 13–17. https://doi.org/10.24176/mjagrotek.v3i1.13088
Apherta, N., Nurcahyani, E., Yulianty, & Wahyuningsih, S. (2025). Analisis Kandungan Klorofil Planlet Anggrek Bulan [Phalaenopsis amabilis (L.) Bl.] Hasil Seleksi Dengan Poly Ethylene Glycol (PEG) 6000 Secara In Vitro. Bioma:Jurnal Mikrobiologi Makasar, 10(1), 126–136.
Arianbach, A. F., Saputra, H. M., & Zasari, M. (2023). Respons Pertumbuhan Planlets Anggrek Phalaenopsis terhadap Pemberian BA dan NAA Selama Aklimatisasi. JURNAL AGROTROPIKA, 22(2), 128. https://doi.org/10.23960/ja.v22i2.7855
Chamara, R. M. S. R., Miyoshi, K., Yukawa, T., Asai, N., & Ogura-Tsujita, Y. (2024). Orchid Mycorrhizal Association of Cultivated Dendrobium Hybrid and Their Role in Seed Germination and Seedling Growth. Microorganisms, 12(6), 1176. https://doi.org/10.3390/microorganisms12061176
Desoky, E.-S. M., Saad, A. M., El-Saadony, M. T., Merwad, A.-R. M., & Rady, M. M. (2020). Plant growth-promoting rhizobacteria: Potential improvement in antioxidant defense system and suppression of oxidative stress for alleviating salinity stress in Triticum aestivum (L.) plants. Biocatalysis and Agricultural Biotechnology, 30, 101878. https://doi.org/10.1016/j.bcab.2020.101878
El-Mageed, T. A. A., El-Mageed, S. A. A., El-Saadony, M. T., Abdelaziz, S., & Abdou, N. M. (2022). Plant Growth-Promoting Rhizobacteria Improve Growth, Morph-Physiological Responses, Water Productivity, and Yield of Rice Plants Under Full and Deficit Drip Irrigation. Rice, 15(1), 16. https://doi.org/10.1186/s12284-022-00564-6
Fitri, H. R., Rahayu, T., Jayanti, G. E., & Agisimanto, D. (2024). Pengaruh Variasi Konsentrasi Indole Butyruc Acid (IBA) Terhadap Pertumbuhan Anggrek (Dendrobium canaliculatum) Pada Media Tumbuh Moss Putih. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 9(2), 38–46. https://doi.org/10.33474/e-jbst.v9i2.507
Hartati, S., Budiyono, A., & Cahyono, O. (2016). Pengaruh NAA Dan BAP Terhadap Pertumbuhan Subkultur Anggrek Hasil Persilangan Dendrobium biggibum X Dendrobium liniale. Caraka Tani: Journal of Sustainable Agriculture, 31(1), 33. https://doi.org/10.20961/carakatani.v31i1.11938
Hidayati, N. Z., Saptadi, D., & Soetopo, L. (2016). Analisis Hubungan Kekerabatan 20 Spesies Anggrek Dendrobium Berdasarkan Karakter Morfologi. Jurnal Produksi Tanaman, 4(4), 291–297.
Jaoudé, R. A., Luziatelli, F., Ficca, A. G., & Ruzzi, M. (2025). Effect of Plant Growth-Promoting Rhizobacteria Synthetic Consortium on Growth, Yield, and Metabolic Profile of Lettuce (Lactuca sativa L.) Grown Under Suboptimal Nutrient Regime. Horticulturae, 11(1), 64. https://doi.org/10.3390/horticulturae11010064
Ng, C. W. W., Yan, W. H., Xia, Y. T., Tsim, K. W. K., & To, J. C. T. (2024). Plant growth-promoting rhizobacteria enhance active ingredient accumulation in medicinal plants at elevated CO2 and are associated with indigenous microbiome. Frontiers in Microbiology, 15, 1426893. https://doi.org/10.3389/fmicb.2024.1426893
Nurbaiti, S., Milasari, A. F., Wibowo, W. A., Nilamsari, E. I., & Rachmawati, D. (2025). Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants. Jurnal Riset Biologi dan Aplikasinya, 7(1), 50–56. https://doi.org/10.26740/jrba.v7n1.p50-56
Prakoso, T., Alpandari, H., Aji, I. K., & Astuti, A. F. T. (2025). The Effect of Plant Growth-Promoting Rhizobacteria (PGPR) Types and Concentration on Soybean (Glycine max L. Merrill) Growth and Yield. AgroTech Food Science, Technology and Environment, 4(2), 90–98. https://doi.org/10.53797/agrotech.v4i2.9.2025
Rahmandhias, D. T., Karyawati, A. S., Hariyono, D., & Maghfoer, M. D. (2024). Effect of plant growth-promoting rhizobacteria (PGPR) on growth and yield of shallots on saline soils. Journal of Degraded and Mining Lands Management, 11(4), 6461–6469. https://doi.org/10.15243/jdmlm.2024.114.6461
Ulinnuha, Z., & Farid, N. (2023). Pertumbuhan Planlet Anggrek Papilionanthe hookeriana x Vanda limbata yang Diinokulasikan Trichoderma sp. Pada Fase Aklimatisasi. AGROSCRIPT: Journal of Applied Agricultural Sciences, 5(2), 115–121. https://doi.org/10.36423/agroscript.v5i2.1265
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255(2), 571–586. https://doi.org/10.1023/A:1026037216893
Wibawati, Z., Sarungallo, A., & Barahima Abbas. (2020). Pertumbuhan Anggrek Grammatophyllum scriptum Asal Kultur In Vitro Pada Berbagai Macam Formulasi Media Tumbuh Berbasis Ampas Sagu. Cassowary, 3(2), 91–100. https://doi.org/10.30862/casssowary.cs.v3.i2.49
Yang, P., Condrich, A., Scranton, S., Hebner, C., Lu, L., & Ali, M. A. (2024). Utilizing Plant Growth-Promoting Rhizobacteria (PGPR) to Advance Sustainable Agriculture. Bacteria, 3(4), 434–451. https://doi.org/10.3390/bacteria3040030
Yasmin, Z. F., Aisyah, S. I., & Sukma, D. (2018). Pembibitan (Kultur Jaringan hingga Pembesaran) Anggrek Phalaenopsis di Hasanudin Orchids, Jawa Timur. Buletin Agrohorti, 6(3), 411. https://doi.org/10.29244/agrob.6.3.411-420
Zakiah, Z., & Turnip, M. (2023). Improving The Growth And Adaptation Of The Black Orchid Plantlet (Coelogyne Pandurata Lindl) In Various Growing Media by Giving Plant Extracts as Biostimulants at The Acclimatization Stage. JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 5(2), 301–310. https://doi.org/10.36378/juatika.v5i2.3113
Zhang, T., Jian, Q., Yao, X., Guan, L., Li, L., Liu, F., Zhang, C., Li, D., Tang, H., & Lu, L. (2024). Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops. Heliyon, 10(10), e31553. https://doi.org/10.1016/j.heliyon.2024.e31553
Apherta, N., Nurcahyani, E., Yulianty, & Wahyuningsih, S. (2025). Analisis Kandungan Klorofil Planlet Anggrek Bulan [Phalaenopsis amabilis (L.) Bl.] Hasil Seleksi Dengan Poly Ethylene Glycol (PEG) 6000 Secara In Vitro. Bioma:Jurnal Mikrobiologi Makasar, 10(1), 126–136.
Arianbach, A. F., Saputra, H. M., & Zasari, M. (2023). Respons Pertumbuhan Planlets Anggrek Phalaenopsis terhadap Pemberian BA dan NAA Selama Aklimatisasi. JURNAL AGROTROPIKA, 22(2), 128. https://doi.org/10.23960/ja.v22i2.7855
Chamara, R. M. S. R., Miyoshi, K., Yukawa, T., Asai, N., & Ogura-Tsujita, Y. (2024). Orchid Mycorrhizal Association of Cultivated Dendrobium Hybrid and Their Role in Seed Germination and Seedling Growth. Microorganisms, 12(6), 1176. https://doi.org/10.3390/microorganisms12061176
Desoky, E.-S. M., Saad, A. M., El-Saadony, M. T., Merwad, A.-R. M., & Rady, M. M. (2020). Plant growth-promoting rhizobacteria: Potential improvement in antioxidant defense system and suppression of oxidative stress for alleviating salinity stress in Triticum aestivum (L.) plants. Biocatalysis and Agricultural Biotechnology, 30, 101878. https://doi.org/10.1016/j.bcab.2020.101878
El-Mageed, T. A. A., El-Mageed, S. A. A., El-Saadony, M. T., Abdelaziz, S., & Abdou, N. M. (2022). Plant Growth-Promoting Rhizobacteria Improve Growth, Morph-Physiological Responses, Water Productivity, and Yield of Rice Plants Under Full and Deficit Drip Irrigation. Rice, 15(1), 16. https://doi.org/10.1186/s12284-022-00564-6
Fitri, H. R., Rahayu, T., Jayanti, G. E., & Agisimanto, D. (2024). Pengaruh Variasi Konsentrasi Indole Butyruc Acid (IBA) Terhadap Pertumbuhan Anggrek (Dendrobium canaliculatum) Pada Media Tumbuh Moss Putih. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 9(2), 38–46. https://doi.org/10.33474/e-jbst.v9i2.507
Hartati, S., Budiyono, A., & Cahyono, O. (2016). Pengaruh NAA Dan BAP Terhadap Pertumbuhan Subkultur Anggrek Hasil Persilangan Dendrobium biggibum X Dendrobium liniale. Caraka Tani: Journal of Sustainable Agriculture, 31(1), 33. https://doi.org/10.20961/carakatani.v31i1.11938
Hidayati, N. Z., Saptadi, D., & Soetopo, L. (2016). Analisis Hubungan Kekerabatan 20 Spesies Anggrek Dendrobium Berdasarkan Karakter Morfologi. Jurnal Produksi Tanaman, 4(4), 291–297.
Jaoudé, R. A., Luziatelli, F., Ficca, A. G., & Ruzzi, M. (2025). Effect of Plant Growth-Promoting Rhizobacteria Synthetic Consortium on Growth, Yield, and Metabolic Profile of Lettuce (Lactuca sativa L.) Grown Under Suboptimal Nutrient Regime. Horticulturae, 11(1), 64. https://doi.org/10.3390/horticulturae11010064
Ng, C. W. W., Yan, W. H., Xia, Y. T., Tsim, K. W. K., & To, J. C. T. (2024). Plant growth-promoting rhizobacteria enhance active ingredient accumulation in medicinal plants at elevated CO2 and are associated with indigenous microbiome. Frontiers in Microbiology, 15, 1426893. https://doi.org/10.3389/fmicb.2024.1426893
Nurbaiti, S., Milasari, A. F., Wibowo, W. A., Nilamsari, E. I., & Rachmawati, D. (2025). Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants. Jurnal Riset Biologi dan Aplikasinya, 7(1), 50–56. https://doi.org/10.26740/jrba.v7n1.p50-56
Prakoso, T., Alpandari, H., Aji, I. K., & Astuti, A. F. T. (2025). The Effect of Plant Growth-Promoting Rhizobacteria (PGPR) Types and Concentration on Soybean (Glycine max L. Merrill) Growth and Yield. AgroTech Food Science, Technology and Environment, 4(2), 90–98. https://doi.org/10.53797/agrotech.v4i2.9.2025
Rahmandhias, D. T., Karyawati, A. S., Hariyono, D., & Maghfoer, M. D. (2024). Effect of plant growth-promoting rhizobacteria (PGPR) on growth and yield of shallots on saline soils. Journal of Degraded and Mining Lands Management, 11(4), 6461–6469. https://doi.org/10.15243/jdmlm.2024.114.6461
Ulinnuha, Z., & Farid, N. (2023). Pertumbuhan Planlet Anggrek Papilionanthe hookeriana x Vanda limbata yang Diinokulasikan Trichoderma sp. Pada Fase Aklimatisasi. AGROSCRIPT: Journal of Applied Agricultural Sciences, 5(2), 115–121. https://doi.org/10.36423/agroscript.v5i2.1265
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255(2), 571–586. https://doi.org/10.1023/A:1026037216893
Wibawati, Z., Sarungallo, A., & Barahima Abbas. (2020). Pertumbuhan Anggrek Grammatophyllum scriptum Asal Kultur In Vitro Pada Berbagai Macam Formulasi Media Tumbuh Berbasis Ampas Sagu. Cassowary, 3(2), 91–100. https://doi.org/10.30862/casssowary.cs.v3.i2.49
Yang, P., Condrich, A., Scranton, S., Hebner, C., Lu, L., & Ali, M. A. (2024). Utilizing Plant Growth-Promoting Rhizobacteria (PGPR) to Advance Sustainable Agriculture. Bacteria, 3(4), 434–451. https://doi.org/10.3390/bacteria3040030
Yasmin, Z. F., Aisyah, S. I., & Sukma, D. (2018). Pembibitan (Kultur Jaringan hingga Pembesaran) Anggrek Phalaenopsis di Hasanudin Orchids, Jawa Timur. Buletin Agrohorti, 6(3), 411. https://doi.org/10.29244/agrob.6.3.411-420
Zakiah, Z., & Turnip, M. (2023). Improving The Growth And Adaptation Of The Black Orchid Plantlet (Coelogyne Pandurata Lindl) In Various Growing Media by Giving Plant Extracts as Biostimulants at The Acclimatization Stage. JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 5(2), 301–310. https://doi.org/10.36378/juatika.v5i2.3113
Zhang, T., Jian, Q., Yao, X., Guan, L., Li, L., Liu, F., Zhang, C., Li, D., Tang, H., & Lu, L. (2024). Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops. Heliyon, 10(10), e31553. https://doi.org/10.1016/j.heliyon.2024.e31553
Diterbitkan sejak
27-06-2026
Rekomendasi Sitasi
Alpandari, H., Widyastuti, W., & Nurlaili, L. (2026). Aplikasi Mikrobia Simbiotik Dalam Meningkatkan Keberhasilan Aklimatisasi Planlet Anggrek. Jurnal Pertanian Terpadu, 14(1), 33-44. https://doi.org/10.36084/jpt.v14i1.730
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