APPLICATION OF SYMBIOTIC MICROBIA IN IMPROVING THE SUCCESS OF ORCHID PLANT ACCLIMATIZATION
Keywords:
Acclimatization, Dendrobium, Symbiotic Bacteria, PGPR
Abstract
Acclimatization is a critical stage in the propagation of orchids produced through tissue culture, as plantlets must adapt from in vitro conditions to the ex vitro environment. The use of symbiotic microorganisms has the potential to enhance acclimatization success by improving nutrient uptake and tolerance to environmental stress. This study aimed to analyze the effect of symbiotic bacterial application on the success of orchid plantlet acclimatization. The experiment employed a Completely Randomized Design (CRD) with five concentrations of symbiotic bacteria: no bacteria (A0), 2% (A1), 4% (A2), 6% (A3), and 8% (A4), each with ten replications. Observed parameters included survival rate, plantlet height, root length, and total chlorophyll content. The results showed that the application of symbiotic bacteria significantly affected all observed parameters. Treatment A3 produced the highest growth and survival rate, reaching 90%.
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References
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Rahmandhias, D. T., Karyawati, A. S., Hariyono, D., & Maghfoer, M. D. (2024). Effect of plant growth-promoting rhizobacteria (PGPR) on growth and yield of shallots on saline soils. Journal of Degraded and Mining Lands Management, 11(4), 6461–6469. https://doi.org/10.15243/jdmlm.2024.114.6461
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Yang, P., Condrich, A., Scranton, S., Hebner, C., Lu, L., & Ali, M. A. (2024). Utilizing Plant Growth-Promoting Rhizobacteria (PGPR) to Advance Sustainable Agriculture. Bacteria, 3(4), 434–451. https://doi.org/10.3390/bacteria3040030
Yasmin, Z. F., Aisyah, S. I., & Sukma, D. (2018). Pembibitan (Kultur Jaringan hingga Pembesaran) Anggrek Phalaenopsis di Hasanudin Orchids, Jawa Timur. Buletin Agrohorti, 6(3), 411. https://doi.org/10.29244/agrob.6.3.411-420
Zakiah, Z., & Turnip, M. (2023). Improving The Growth And Adaptation Of The Black Orchid Plantlet (Coelogyne Pandurata Lindl) In Various Growing Media by Giving Plant Extracts as Biostimulants at The Acclimatization Stage. JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 5(2), 301–310. https://doi.org/10.36378/juatika.v5i2.3113
Zhang, T., Jian, Q., Yao, X., Guan, L., Li, L., Liu, F., Zhang, C., Li, D., Tang, H., & Lu, L. (2024). Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops. Heliyon, 10(10), e31553. https://doi.org/10.1016/j.heliyon.2024.e31553
Apherta, N., Nurcahyani, E., Yulianty, & Wahyuningsih, S. (2025). Analisis Kandungan Klorofil Planlet Anggrek Bulan [Phalaenopsis amabilis (L.) Bl.] Hasil Seleksi Dengan Poly Ethylene Glycol (PEG) 6000 Secara In Vitro. Bioma:Jurnal Mikrobiologi Makasar, 10(1), 126–136.
Arianbach, A. F., Saputra, H. M., & Zasari, M. (2023). Respons Pertumbuhan Planlets Anggrek Phalaenopsis terhadap Pemberian BA dan NAA Selama Aklimatisasi. JURNAL AGROTROPIKA, 22(2), 128. https://doi.org/10.23960/ja.v22i2.7855
Chamara, R. M. S. R., Miyoshi, K., Yukawa, T., Asai, N., & Ogura-Tsujita, Y. (2024). Orchid Mycorrhizal Association of Cultivated Dendrobium Hybrid and Their Role in Seed Germination and Seedling Growth. Microorganisms, 12(6), 1176. https://doi.org/10.3390/microorganisms12061176
Desoky, E.-S. M., Saad, A. M., El-Saadony, M. T., Merwad, A.-R. M., & Rady, M. M. (2020). Plant growth-promoting rhizobacteria: Potential improvement in antioxidant defense system and suppression of oxidative stress for alleviating salinity stress in Triticum aestivum (L.) plants. Biocatalysis and Agricultural Biotechnology, 30, 101878. https://doi.org/10.1016/j.bcab.2020.101878
El-Mageed, T. A. A., El-Mageed, S. A. A., El-Saadony, M. T., Abdelaziz, S., & Abdou, N. M. (2022). Plant Growth-Promoting Rhizobacteria Improve Growth, Morph-Physiological Responses, Water Productivity, and Yield of Rice Plants Under Full and Deficit Drip Irrigation. Rice, 15(1), 16. https://doi.org/10.1186/s12284-022-00564-6
Fitri, H. R., Rahayu, T., Jayanti, G. E., & Agisimanto, D. (2024). Pengaruh Variasi Konsentrasi Indole Butyruc Acid (IBA) Terhadap Pertumbuhan Anggrek (Dendrobium canaliculatum) Pada Media Tumbuh Moss Putih. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 9(2), 38–46. https://doi.org/10.33474/e-jbst.v9i2.507
Hartati, S., Budiyono, A., & Cahyono, O. (2016). Pengaruh NAA Dan BAP Terhadap Pertumbuhan Subkultur Anggrek Hasil Persilangan Dendrobium biggibum X Dendrobium liniale. Caraka Tani: Journal of Sustainable Agriculture, 31(1), 33. https://doi.org/10.20961/carakatani.v31i1.11938
Hidayati, N. Z., Saptadi, D., & Soetopo, L. (2016). Analisis Hubungan Kekerabatan 20 Spesies Anggrek Dendrobium Berdasarkan Karakter Morfologi. Jurnal Produksi Tanaman, 4(4), 291–297.
Jaoudé, R. A., Luziatelli, F., Ficca, A. G., & Ruzzi, M. (2025). Effect of Plant Growth-Promoting Rhizobacteria Synthetic Consortium on Growth, Yield, and Metabolic Profile of Lettuce (Lactuca sativa L.) Grown Under Suboptimal Nutrient Regime. Horticulturae, 11(1), 64. https://doi.org/10.3390/horticulturae11010064
Ng, C. W. W., Yan, W. H., Xia, Y. T., Tsim, K. W. K., & To, J. C. T. (2024). Plant growth-promoting rhizobacteria enhance active ingredient accumulation in medicinal plants at elevated CO2 and are associated with indigenous microbiome. Frontiers in Microbiology, 15, 1426893. https://doi.org/10.3389/fmicb.2024.1426893
Nurbaiti, S., Milasari, A. F., Wibowo, W. A., Nilamsari, E. I., & Rachmawati, D. (2025). Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants. Jurnal Riset Biologi dan Aplikasinya, 7(1), 50–56. https://doi.org/10.26740/jrba.v7n1.p50-56
Prakoso, T., Alpandari, H., Aji, I. K., & Astuti, A. F. T. (2025). The Effect of Plant Growth-Promoting Rhizobacteria (PGPR) Types and Concentration on Soybean (Glycine max L. Merrill) Growth and Yield. AgroTech Food Science, Technology and Environment, 4(2), 90–98. https://doi.org/10.53797/agrotech.v4i2.9.2025
Rahmandhias, D. T., Karyawati, A. S., Hariyono, D., & Maghfoer, M. D. (2024). Effect of plant growth-promoting rhizobacteria (PGPR) on growth and yield of shallots on saline soils. Journal of Degraded and Mining Lands Management, 11(4), 6461–6469. https://doi.org/10.15243/jdmlm.2024.114.6461
Ulinnuha, Z., & Farid, N. (2023). Pertumbuhan Planlet Anggrek Papilionanthe hookeriana x Vanda limbata yang Diinokulasikan Trichoderma sp. Pada Fase Aklimatisasi. AGROSCRIPT: Journal of Applied Agricultural Sciences, 5(2), 115–121. https://doi.org/10.36423/agroscript.v5i2.1265
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255(2), 571–586. https://doi.org/10.1023/A:1026037216893
Wibawati, Z., Sarungallo, A., & Barahima Abbas. (2020). Pertumbuhan Anggrek Grammatophyllum scriptum Asal Kultur In Vitro Pada Berbagai Macam Formulasi Media Tumbuh Berbasis Ampas Sagu. Cassowary, 3(2), 91–100. https://doi.org/10.30862/casssowary.cs.v3.i2.49
Yang, P., Condrich, A., Scranton, S., Hebner, C., Lu, L., & Ali, M. A. (2024). Utilizing Plant Growth-Promoting Rhizobacteria (PGPR) to Advance Sustainable Agriculture. Bacteria, 3(4), 434–451. https://doi.org/10.3390/bacteria3040030
Yasmin, Z. F., Aisyah, S. I., & Sukma, D. (2018). Pembibitan (Kultur Jaringan hingga Pembesaran) Anggrek Phalaenopsis di Hasanudin Orchids, Jawa Timur. Buletin Agrohorti, 6(3), 411. https://doi.org/10.29244/agrob.6.3.411-420
Zakiah, Z., & Turnip, M. (2023). Improving The Growth And Adaptation Of The Black Orchid Plantlet (Coelogyne Pandurata Lindl) In Various Growing Media by Giving Plant Extracts as Biostimulants at The Acclimatization Stage. JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 5(2), 301–310. https://doi.org/10.36378/juatika.v5i2.3113
Zhang, T., Jian, Q., Yao, X., Guan, L., Li, L., Liu, F., Zhang, C., Li, D., Tang, H., & Lu, L. (2024). Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops. Heliyon, 10(10), e31553. https://doi.org/10.1016/j.heliyon.2024.e31553
Published
27-06-2026
How to Cite
Alpandari, H., Widyastuti, W., & Nurlaili, L. (2026). APPLICATION OF SYMBIOTIC MICROBIA IN IMPROVING THE SUCCESS OF ORCHID PLANT ACCLIMATIZATION. Jurnal Pertanian Terpadu, 14(1), 33-44. https://doi.org/10.36084/jpt.v14i1.730
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